| RNASEN

Drosha is a Class 2 ribonuclease III enzyme that is encoded by the DROSHA (formerly RNASEN) gene. It is the primary nuclease that executes the initiation step of miRNA processing in the nucleus. It works closely with DGCR8 and in correlation with Dicer. It has been found significant in clinical knowledge for cancer prognosis and HIV-1 replication.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.19	1.61	1.05	0.66	0.43	0.29	0.21	0.07
Corrected OD	2.12	1.54	0.98	0.59	0.36	0.22	0.14	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human RNASEN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	89-105	102
Cell Lysates(n=8)	96-108	101

 

| Linearity

Samples were spiked with high concentrations of Human RNASEN and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-96	92-110
	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
	Average (%)	100	108